Before the experiment: Basic protection

Experimental personnel are prone to blood/body fluid contamination or being stabbed by instruments or needles during operation, posing safety hazards. Therefore, self-protection must be taken into account. Therefore, the most basic protection should be taken before the experiment: wear necessary protective equipment such as gloves, masks, hats, shoe covers, and work clothes, and if necessary, wear rubber boots and goggles (or face shields).

In the experiment: Accident prevention (common types of accidents in animal experiments)

The experimenter was stabbed or cut, and was bitten by an animal

(1) Immediately terminate the experiment and return the experimental animals to their cages.

(2) Deal with the wound immediately.

① If the bite is mild, with no bleeding or minimal bleeding, rinse the injured area repeatedly with clean water or soapy water as soon as possible, try to remove any liquid from the animal's mouth, and disinfect the skin or wound area with iodine or 75% alcohol.

② If there is bleeding, squeeze the wound with your hands to allow blood to flow out, and rinse the wound repeatedly with running water for 1-2 minutes. Then use iodine or 75% alcohol to thoroughly disinfect the skin or wound area (with some force, not just gentle wiping), and wrap the wound with gauze or band aids to stop bleeding. When necessary, injection of tetanus antitoxin and use of antibiotics can be considered to prevent other bacterial infections (commonly used pestis, hemorrhagic fever and tetanus triple vaccine)

When the wound is large and there is a lot of bleeding, it should be taken to the hospital for treatment.

In addition to the above-mentioned wound treatment, it is necessary to have a detailed understanding of the source and vaccination status of the batch of animals, and decide whether to inject rabies vaccine, anti serum, or immunoglobulin depending on the degree of bite.

(3) Make relevant records: Fill out the experimental accident record form, including the name of the injured person, time and location of injury, nature of the wound, and whether there is bleeding. When animal bites occur, detailed records should be kept of the type of animal that caused the injury, the source of the animal, the animal grade, the purpose or name of the experiment, and the reagents used.

Animal Escape

(1) Immediately stop the experiment and capture the animals. Before capturing the escaped animal, laboratory personnel are not allowed to leave the animal laboratory to prevent animals from escaping into public areas.

(2) If small rodents escape before the experiment, long handled tweezers should be used immediately to quickly and accurately clamp the base of the animal's tail. After capturing the animal, it should be placed in a cage and wait for the animal's emotions to stabilize before conducting the experiment; If the animal escapes quickly and is difficult to grasp with tweezers, a specialized animal capture net can be used for capture.

(3) In case of animal escape during the experiment, the escape route of animals and the blood and secretions splashed by animals should be wiped and disinfected, and secondary disinfection and sterilization should be organized.

(4) Rodent escape often occurs, and in general, experiments can continue after capturing the animals, but only within the laboratory; After animals escape from the laboratory, even if they are captured, they should not be used again in principle.

After the experiment: Properly dispose of experimental waste

After the experiment is completed, consideration should also be given to the harmless treatment of waste liquids, solid materials, animal carcasses, specimens, and other materials generated during the animal experiment process. There are two methods of treatment: chemical methods and physical methods. Chemical methods involve disinfecting with chemical reagents, while physical methods often involve high-temperature sterilization and incineration.

The laboratory should be equipped with dedicated garbage bins and animal carcass bags, which should be classified and stored by experimenters according to operating procedures. It is strictly prohibited to mix and store animal carcasses with experimental waste (needles, blades, syringes, gloves, padding, animal blood, tissue fluid, and wiping materials, etc.).